WGS of Phaseolus lunatus: sample G26703 - SRA

SRX16850495: WGS of Phaseolus lunatus: sample G26703
1 ILLUMINA (Illumina NovaSeq 6000) run: 29M spots, 8.8G bases, 2.7Gb downloads

Design: The seeds were obtained from the International Center for Tropical Agriculture (CIAT) (See Supplementary table 7 for more detail). Young trifoliate leaves from two-week-old seedlings were collected and frozen with liquid nitrogen. Based on the DNA integrity and concentration requirements of Illumina sequencing technology, DNA extraction was performed using the extraction method developed by Vega-Vela & Snchez (2011). The Illumina library used 1.0 g of DNA according to a NEBNext DNA Library Preparation Kit following the manufacturer's recommendations (New England BioLabs, Ipswich, MA, USA). Genomic DNA was fragmented to 350 bp in size, fragments ligated to NEBNext adapters and enriched by PCR. Library size distribution was analyzed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified by real-time PCR. Libraries were sequenced on an Illumina HiSeq platform (Illumina, San Diego, CA, USA) using a 150 paired-end run (2 150 bases) and an insert size of 450 bp.

Submitted by: Universidad de los Andes

Study: Phaseolus lunatus genomic resources

PRJNA596114 • SRP238028 • All experiments • All runs

show Abstracthide Abstract

Genome assembly and variability of Lima bean (Phaseolus lunatus)

Sample: Phaseolus lunatus leaf tissue for WGS DNA sequencing. Accession G26703

SAMN30076410 • SRS14455237 • All experiments • All runs

Organism: Phaseolus lunatus

Library:

Name: WGS-G26703

Instrument: Illumina NovaSeq 6000

Strategy: WGS